MicroRNA profiles under ischemia-induced neovascularization were measured, the 3 downregulated miRNAs - 31, 150, and 184 were studied further. Injection of these miRNAs reduced angiogenesis in retinal cells, injection of-31, and -150 had the same effect on choroidal cells. Reporter assays validated targets for miR-31 (Pdgfb, Hif1a), and miR-150 (Pdgfb, Vegf) - all proangiogenic. Frizzled4 was identified bioinformatically as a target of miR-184. Induced VEGF expression, blockaded in AMD treatment, suppressed miR-150 while VEGF levels were also reduced by miR-31, possibly through HIF-1alpha, a transcriptional activator of VEGF.
[lab][cta]
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