The miRNA profile of human serum was analysed. Harsh storage conditions and RNAse A digestion which destroyed longer RNA species was shown to have little effect on miRNA levels. Sequencing showed that miRNAs made up the largest part of small polynucleotide species, with about 190 miRNAs referenced. Measured expression was seen to be similar across individuals. Analysis of diseased subjects showed large changes in the profile (NSC-lung carcinoma = 28 miRNA missing, 63 novel) compared to healthy individuals. Serum samples from patients with colorectal cancer, and diabetes showed further differentiation of miRNA profiles. The particular miRNAs elevated came contained members previously associated with the diseases mentioned.
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